ITO - In the Air Degign Challenge - Portable Field Tester #5

RE: ITO - In the Air Degign Challenge - Portable Field Tester #5

dwinhold — Wed, 17 Dec 2014 05:37:30 GMT

My original speed of 18000 rpm - RFC 27167 would have accomplished separation in around 2 minutes. the vials are small, 1.5 ml and rated for up to 30000 RFC. I have also got a friend (who owns a machine shop) to donate his time and make me the rotor from a solid block of aluminum, balance and fit everything. We will be using the lower speed and more time for separation. Below are the centrifuge calculations:

RCF=(RPM/1000)^2 x r x 1.118

RE: ITO - In the Air Degign Challenge - Portable Field Tester #5

dwinhold — Wed, 17 Dec 2014 05:16:50 GMT

I really like the feedback, thank you to everyone. Safety is first, the centrifuge will be enclosed and housed safely, nothing is worth risk taking.

My research on speed, g-force and time required:

Time for bacteria separation with below calculations - 15 + minutes

RPM - 9500

Radius from center to tip of vial - 75mm (3")

G-force or RFC - 7567

RE: ITO - In the Air Degign Challenge - Portable Field Tester #5

michaelwylie — Tue, 16 Dec 2014 20:13:22 GMT

I don't know anything about centrifuges, but for my own curiosity, how much energy are we talking about? The glass vials will explode against the wall or the entire machine will explode?

RE: ITO - In the Air Degign Challenge - Portable Field Tester #5

jw0752 — Tue, 16 Dec 2014 05:24:42 GMT

Dear Dale and Chrystal,

Be careful with the centrifuge especially if your vials are glass. I have repaired centrifuges and about 50% of the machine is devoted to protection and safety. At 18,000 RPM you have a dangerous amount of energy stored in the moving parts. Another aspect that is often missed is the balance that the wheel must have at 18,000 RPM to keep from shaking apart. You may want to talk to a Veterinary Clinic or a Medical Lab to see if they would separate your samples for you.

John

RE: ITO - In the Air Degign Challenge - Portable Field Tester #5

pmohan — Tue, 16 Dec 2014 03:13:29 GMT

Great...I want to see this field sensor and microscope.. awaiting the next update