So I am currently working with an arduino nano and I just built a prototype PCB for the system I was working with on a breadboard previously. After checking the PCB connection many times I finally tested it with my nano. Everything seems to be working as expected. However, now that I am further developing the sketch I want to run I cannot upload and am getting the error shown below. This error only occurs when seated in the headers on my PCB. If I take the nano off the board then everything is fine and I can upload my sketch. What are some potential issues with my PCB that could be causing this? Is it possible to answer this without seeing the board? Is it safe to keep using the PCB as is and just unseat the nano every time I want to update the sketch?